首先，我们应该尊重审稿人，可以毫不夸张地说，在你的文章出版之前，甚至出版之后，没有几个人会像审稿人那样仔细体会和分析你的文章。
因此，我们应该充满感激、也认真思考和领会他们所提出的每一条意见，甚至是近乎苛刻的意见，但也不卑不亢。对于审稿人提出的意见，切忌“硬碰硬”，回复技巧很重要。
Thank you for your letter and for the reviewers’ comments concerning our manuscript entitled “文章题目”. These comments are all valuable and very helpful for revising and improving our paper, as well as the important guiding significance to our researches. We have studied comments carefully and have made corrections which we hope meet with approval. （你的提议太有用了，我论文的质量蹭蹭蹭就上去了！我们相当仔细研究了你的建议之类的话）The responds to the reviewer’s comments are as follows: …….
回复：As for the referee’s concern, we have added scale bars to the Figures.
回复：As for the referee’s concern, we have indicated a significant difference with asterisk in figures.
回复：We are very sorry for the mistakes in this manuscript and inconvenience they caused in your reading. The manuscript has been thoroughly revised and edited by a native speaker, so we hope it can meet the journal’s standard. Thanks so much for your useful comments.
回复：As for the referee’s concern, the full descriptions of the abbreviations like IHC, MTT etc have been supplemented in the revised manuscript.
回复：We are very sorry for the mistake. Lentivirus-transduced cells were washed twice with PBS, and suspended in a lysis buffer (2% mercaptoethanol, 20% glycerol, and 4% SDS, in 100 mM Tris-HCl buffer, pH 6.8). The information for the lysis buffer has been corrected in the materials and methods.
技巧3：补充实验，耗时耗力，如果不补，只能argue
Tips：如果你真的不想补，或者正常细胞株，你也买不到，还是查一查正常细胞株的名字，告诉审稿人你确实是去努力过，只是事与愿违啊！
回复：Indeed, it will be more convincing if we get a comparative assessment on normal keratinocytes. However, the normal skin cell line TE 353.SK that we recently obtained could not be cultured stably under the condition of our lab at this time. We are also collecting human tissue samples from patients with primary melanoma and benign nevi. Based on our data, XXX expression will be analyzed in clinical samples by immunohistochemistry, and the correlation between XXX expression and the prognosis of melanoma will be determined in our subsequent investigations. Therefore, the referee’s concern is of importance for our further study, and we will show the results in our next paper for XXX.
Tips：补充动物实验，是审稿人最爱发的一招，其实一点不令人意外。既然躲不过，就要见招拆招，如果能补上，当然是给了漂亮的回马枪；要是没有条件补，或者实验来不及，也要尽量说的委婉点，假装你确实努力了，是审稿人给的时间太短了，我根本来不及啊！
回复：We are appreciative of the reviewer’s suggestion. Indeed, it will be more profound if we get the relevant results in vivo. We have purchased 10 thymus- BALB/c nude mice (female, age 4-6 weeks) from the Animal Center of Chinese Academy of Medical Sciences. Osteosarcoma xenografts were established in nude mice according to a previous report (Gao et al., 2009) （我们去买了若干只小鼠，按照文献的方法操作）. Briefly, control lentivirus or XXX gene shRNA-expressing lentivirus infected XXX cells (1×105) were suspended in 0.1 ml PBS and then injected subcutaneously into the proximal tibia of each anesthetized nude mice (n=5 in each group). Unfortunately, the xenografts could not grow after inoculation for three weeks （满怀欣喜的接种到动物身上，然而实验这东西，哪能一遍就成呢，我们做了一遍，结果没能成瘤，实在是悲剧了）. The limitation of technical condition may be the main reason. Also, we doubt that the cell viability could be insufficient to initiate osteosarcoma tumorigenesis in vivo due to the long-distance transport. Therefore, we seek for the editor’s tolerance and understanding. Many thanks for your kind help!
然而，补充实验，还是逃不过的一劫，究竟是申请延期，再老老实实把实验做完，还是想投机取巧，argue一下，就看自己的运气了。一般情况，可以对照审稿人的意见综合权衡。高分文章，最好还是该补的全补了，低分文章，如果你觉得有80%的把握，完全可以凭你的劲爆人品成功拿下审稿人。
目前几乎所有期刊都会对来稿进行论文查重，据了解英文投稿目前有95%以上的期刊是使用iThenticate检测来稿，投稿前检测请认准iThenticate中文网站：http://www.ithenticate.com.cn/，安全可靠，投稿无忧！
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